Email:email@example.com Phone: 310-825-6430 Address: A-420 JLNRC Research Interests: Structural organization of biological molecules (channels and pumps) by the use of electron microscopy and computer image processing.
ntercellular junctions and communication
A fundamental property of cells organized in tissues is their ability to communicate with ech other via highly specialized areas of plasma membrane contact, called junctions. We are studying the gap junction and the chemical synapse, two specialized junctions that mediate electrical and chemical transmission, respectively. The gap junction is composed of specialized channels containig small hydrophilic pores that span the two plasma membranes and the intervening extracellular space (the cell-to-cell channel). We are investigating the high resolution structure of the channels as well as the mechanisms of assembly, insertion and retrieval of the connexin proteins which form them. The chemical synapse is composed of a presynaptic neuron filled with small diameter vesicles that contain high concentration of neurotransmitters and a postsynaptic neuron with receptors. We are studying the mechanisms by which the synaptic vesicles attach ("dock") to the presynaptic membrane. We use structural (electron microscopy), biochemical and electrophysiological techniques in an attempt to identify, purify and characterize the channels and transporters of both junctions in an effort to further understand the mechanisms mediating cell communication.
Zampighi, G.A, Kreman, M., Lanzavecchia, S., Turk, E., Eskandari, E., Zampighi, L., Wright, E.M.
Structure of functional AQP0 channels in phospholipid bilayers J. Mol. Biol
Distribution of connexin50 channels and hemichannels in lens fibers: a structural approach Cell Commun Adhes
Zampighi GA, Eskandari S, Hall JE, Zampighi L, Kreman M
Micro-domains of AQPO in lens equatorial fibers Exp Eye Res
Eskandari S, Zampighi GA, Leung DW, Wright EM, Loo DD
Inhibition of gap junction hemichannels by chloride channel blockers J Membr Biol
Mischel PS, Umbach JA, Eskandari S, Smith SG, Gundersen CB, Zampighi GA
Nerve growth factor signals via preexisting TrkA receptor oligomers Biophys J
Eskandari, S., Kreman, M., Kavanaugh, M.P., Wright, E.M., Zampighi, G.A
A pentameric assembly of a neuronal glutamate transporter (EAAT3) Proc. Natl. Acad. Sci
Turk E, Kim O, le Coutre J, Whitelegge JP, Eskandari S, Lam JT, Kreman M, Zampighi G, Faull KF, Wright EM
Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransporters J Biol Chem
Zampighi GA, Eskandari S, Kreman M
Epithelial organization of the mammalian lens Exp Eye Res
Zampighi, G.A., Loo, D.D.F., Kreman, M., Eskandari, S., and Wright, EM
Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes J. Gen Physiol
Eskandari S, Snyder PM, Kreman M, Zampighi GA, Welsh MJ, Wright EM
Number of subunits comprising the epithelial sodium channel J Biol Chem
Calderon V, Lazaro A, Contreras RG, Shoshani L, Flores-Maldonado C, Gonzalez-Mariscal L, Zampighi G, Cereijido M
Tight junctions and the experimental modifications of lipid content J Membr Biol
Eskandari S, Wright EM, Kreman M, Starace DM, Zampighi GA
Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy Proc Natl Acad Sci U S A
Wright EM, Hirsch JR, Loo DD, Zampighi GA
Regulation of Na+/glucose cotransporters J Exp Biol
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Konig N, Zampighi GA, Butler PJ
Characterisation of the major intrinsic protein (MIP) from bovine lens fibre membranes by electron microscopy and hydrodynamics J Mol Biol
Zampighi GA, Fisher RS
Polyhedral protein cages encase synaptic vesicles and participate in their attachment to the active zone J Struct Biol
Panayotova-Heiermann M, Eskandari S, Turk E, Zampighi GA, Wright EM
Five transmembrane helices form the sugar pathway through the Na+/glucose cotransporter J Biol Chem
Chandy G, Zampighi GA, Kreman M, Hall JE
Comparison of the water transporting properties of MIP and AQP1 J Membr Biol
Konig, N. and Zampighi, G.
Purification of bovine lens cell-to-cell channels composed of connexin44 and connexin50 J. Cell. Sci
Mandel, L.J., Bacallao, R. and Zampighi, G.
Uncoupling of the molecular "fence" and paracellular "gate" functions in epithelial tight junctions Nature